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QTube Introduction Video
December 28, 2009

This video introduces QTube, and walks you through the benefits it provides. It can help you learn different procedures, solve your problems, and make life a lot easier.

QTube provides easy access to a multitude of imaging techniques and procedures, and real-time, practical assistance to those who need “quick and easy” access to life science digital imaging resources.The QTube video playlist displays here when your version of Flash Player is updated.
thumbnail preview of videoQTube Introduction Video

This video introduces QTube, and walks you through the benefits it provides. It can help you learn different procedures, solve your problems, and make life a lot easier.

thumbnail preview of videoAcquire and Multichannel Capture in Metamorph

Part 1 of "Using the Multi Dimensional Acquisition in Metamorph." Demonstrates how to set up complex multi-dimensional imaging acquisition experiments. Control timelapse, stage, wavelengths, z series, stream acquisitions or even run journals.

thumbnail preview of videoAcquiring Color Images in Image-Pro Plus and QCapture Pro

This shows how to control color cameras and adjust for color balance to create the best colour image within Image-Pro Plus and QCapture Pro software.

thumbnail preview of videoAligning a Sequence by an Individual Track, then Tracking Objects

This movie uses a pre-recorded macro to align a sequence by a single object track in Image-Pro Plus. The individual objects in the sequence can then be tracked more accuratley in Image-Pro Plus.

thumbnail preview of videoColor Overlay

Have different color (λ) images that you wish to overlay in QCapture Pro? Use this easy to follow tutorial on how to create your color overlay images and how to make them look like your sample.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

thumbnail preview of videoConfiguring AutoQuant for Batch Deconvolution

This video demonstrates how to use the batch processing function of AutoQuant, allowing you to configure the software to perform multiple deconvolutions at pre-defined times.

thumbnail preview of videoControl Shutters and LED's Using Parallel Port Cables

This shows how set up and control a parallel port device such as a shutter or LED light source inside Image-Pro Plus.

thumbnail preview of videoControlling 2 Cameras in Metamorph

This shows how to control Photometrics cameras inside Metamorph.

thumbnail preview of videoControlling Multi-Dimensional Acquisition Using AFA Module

This movie shows to use the AFA module within Image-Pro Plus.

thumbnail preview of videoControlling Stage Positions in Metamorph

Part 4 of "Using the Multi Dimensional Acquisition in Metamorph." Demonstrates how to set up complex multi-dimensional imaging acquisition experiments. Controlling multiple stage positions.

thumbnail preview of videoCreating a Sorted Objects Sequence in Image-Pro

This movie shows you how to use a simple macro to sort counted objects into a sequence in Image-Pro Plus.

thumbnail preview of videoDownloading and Installing the Thesycon Stack

This movie shows you where to find and how to install the Thesycon stack.

This is a driver for your PCI card and is required by the latest version of QImaging drivers.

thumbnail preview of videoDual-View™: Basic Control & Software

If you’re using a Dual-View™ or wavelength splitting device and have trouble aligning it just right, this tutorial will guide you along and help you do it right, using Image Pro Plus.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

thumbnail preview of videoDual-View™: Intensity Over Time

Trying to take a live intensity measurement over time, using a Dual-View™ or wavelength splitting device? Follow along as we show you the steps for making the measurement effortlessly.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

thumbnail preview of videoDynamic Range Calculator

The Dynamic Range calculator provides you with a helpful tool which allows you to calculate the maximum dynamic range of your camera. The Dynamic Range describes the ratio between the smallest and largest possible values the camera can quantitatively detect.

  • Refer to the Datasheet or the Manufacturer's website to find the Maximum Linear Full Well Capacity.
  • Using the Read Noise calculator, enter the value of your read noise.

Related Links:

http://www.evolve-emccd.com/calculators/dynamicrange.php

 

http://www.evolve-emccd.com/whitepapers/dynamicrange.php

thumbnail preview of videoHardware Required for a Deconvolution System Using Image-Pro Plus

This shows the differnet components required to set up a deconvolution system using a QImaging camera, an objective mover and Image-Pro Plus software with the IO-Pro module.

thumbnail preview of videoHardware Set Up for Synchronized Streaming

This shows how to connect 2 Photometrics cameras with the StreamPix pulse generator.

thumbnail preview of videoHow To Use Triggering With Your Q Camera

A demonstration on how to connect the external triggering box to your QImaging camera, including:

  • Overview of triggering modes and options
  • Configuring QCapture Pro for external triggering
  • And an example triggering diagram

You may also be interested in:

thumbnail preview of videoImage Warping Macro of Image-Pro Plus

This movies shows you how to use the Warping Macro in Image-Pro Plus. This macro is found on the Media Cybernetics Solution Zone Solution Zone # 595. This macro allows you correct images for warping and perspective issues.

thumbnail preview of videoImage-Pro Plus: 3D Surface Inspector Acquisition and Visualization

Create calibrated and quantitative topographical maps and surface renderings with or without automated focus control.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: 3D Viewer

View 3D images using the 3D Viewer.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: Align Multiple Images

Use the image alignment tool to correct for stereo, rotational, and other types of shifts.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: Apply Calibration Marker

Easily apply marker bars to your calibrated images.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: Basic Deconvolution

Remove haze from your images using the built-in deconvolution tools.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: Calibration

Calibrate to receive the correct measurement data.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: Create a Custom Toolbar

Create a customized toolbar to fit your workflow.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: Editing Thresholds & Background Correction

Learn how to edit your threshold operations for improved accuracy & object selection.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: Intensity Over Time

Measure intensity over time.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: Live EDF

Create in-focus composite images live - without an automated focus drive.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: Live Tiling

Tile large images live – without an automated stage.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: Prepare, Threshold, and Track 2D Moving Objects

A brief tutorial on 2D Tracking using Image-Pro Plus.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: Record a Macro

Record a macro and automate your processes.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: Stitch Multiple Images

Use the image stitching tools to combine multiple image fields.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: Threshold & Measure Objects

Use the thresholding tool to segment and count objects in your images.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: Threshold Color Images

Threshold and segment colored objects in your images.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoImage-Pro Plus: Tint and Merge

Use Image-Pro Plus to tint and merge color channels.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

 

For more information on Image-Pro Plus, visit www.mediacy.com.

thumbnail preview of videoInstalling a QImaging Camera with the New Driver

Use this tutorial for a speedy, problem-free install of the QCam driver.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

thumbnail preview of videoIntalling a FireWire PCI Card

This Video demonstrates how to install a PCI or PCI Express Firewire card in your PC. QImaging and Photometrics always provide PCI cards with cameras and it is advised you install and use these cards for the best results.

thumbnail preview of videoMaking Live Intensity Measurements using a Dual-View™

If you’re using a Dual-View™ or a wavelength splitting device, let this tutorial guide you through the ins and outs of taking live intensity measurements with Image Pro Plus.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

thumbnail preview of videoObjective Imaging Turboscan

This movie shows you the Objective Imaging Turboscan function operating as a plug-in to Image-Pro Plus. This plug-in allows rapid slide scaning and has advanced memory handling features allowing you to view extremley large datasets.

thumbnail preview of videoPerforming a Basic 3D Deconvolution in AutoQuant

This video shows you how to configure AutoQuant for a basic 3D deconvolution on a Z-stack of images.

thumbnail preview of videoPerforming a Firework Track in Image-Pro Plus

This movie demonstrates how to use the 'Firework track' macro in Image-Pro Plus. This macro is available at Media Cybernetics Solution Zone and allows a user to create a cumulative track throughout a sequence and explains where this might be useful.

thumbnail preview of videoPerforming Ratio Analysis in Image-Pro

This movie shows you how to quickly perform Ratiometric analysis of images in Image-Pro and measure intensity over time. It also shows you a macro that gives an average intensity of an image stack, which can then also be used for ratiometric analysis.

thumbnail preview of videoPhotometrics’ Evolve EMCCD Background Event Reduction Technology™

"BERT" identifies pixels that are likely to contain spurious even data and then makes corrections, if desired.

 

Related Links:

www.evolve-emccd.com/bert

www.evolve-emccd.com/technotes/evolve512-advancedfeatures.php

thumbnail preview of videoPhotometrics’ Evolve EMCCD Black-Lock™/Top-Lock™ Technology

This intensity-filtering tool narrows visualization to the intensity range of the image features where the user is most interested.

 

Related Links:

www.evolve-emccd.com/bltl

www.evolve-emccd.com/technotes/evolve512-advancedfeatures.php

thumbnail preview of videoPhotometrics’ Evolve EMCCD Quant-View™ Technology

Allows camera to read out pixel values in terms of electrons measured, thereby enabling user to be completely quantitative. The Evolve calculates Analog Gain (Gain State), Electron Multiplication Gain (EM Gain) and Bias level.

 

Related Links:

www.evolve-emccd.com/quantview

www.evolve-emccd.com/technotes/evolve512-advancedfeatures.php

thumbnail preview of videoPhotometrics’ Evolve EMCCD Rapid-Cal™ Technology

Quickly and easily perform EM calibration process in 3 minutes. Its accuracy is equivalent to performing EM-Gain mapping by taking over 5000 points on the EM Gain curve and calibrating manually.

 

Related Links:

www.evolve-emccd.com/rapidcal

www.evolve-emccd.com/technotes/evolve512-advancedfeatures.php

thumbnail preview of videoPhotometrics’ Evolve EMCCD Vari-Bit™ Selectable Bit Depth (8-16 bits)

This technology improves data by matching digitization bit-depth to actual intrascene dynamic range

 

Related Links:

www.evolve-emccd.com/varibit

www.evolve-emccd.com/technotes/evolve512-advancedfeatures.php

thumbnail preview of videoProper Detection of Photometrics LVDS Camera (Windows Vista 32 bit)

Solving issues seen when running Photometrics LVDS cameras on a Vista 32 operating system.

 

Instructions available as pdf:

Ensuring your Photometrics LVDS camera is properly detected in Windows Vista (32 bit) (.pdf)

thumbnail preview of videoQCapture Pro: Guide to Camera Control

Learn the intricacies of QCapture Pro as we show you how to use this versatile program with your camera and the different controls it allows you.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

thumbnail preview of videoQimaging - What's in the Box

This is a quick movie to show you exactly what components are included in a QImaging box.

thumbnail preview of videoQImaging Magnetic C-Mounts

A Quick Movie to show you the Q-Imaging Magnetic C-Mounts, these mounts allow you to quickly switch between C-Mount Cameras, removing the need for screwing each camera on each time

thumbnail preview of videoQuad-View Control and Software Alignment

This movie shows how we set up and acquire images a Quad-View unit and then post acquisition software register the different channels.

thumbnail preview of videoRead Noise Calculator

The Read Noise calculator provides you with a helpful tool which allows you to calculate the read noise of your camera. The read noise is a measure of the uncertainty associated with each image acquisition and is a function of the camera system.

  • Enter the Standard Deviation of the pixels in the image.
  • Enter the Gain value of the camera (e-/ADU)

If you do not have the gain value for your camera, use our Mean Variance (Gain) Calculator.

 

Related Links:

http://www.evolve-emccd.com/calculators/readnoise.php

 

http://www.evolve-emccd.com/whitepapers/readnoise.php

thumbnail preview of videoReviewing Data in Metamorph

Part 5 of "Using the Multi Dimensional Acquisition in Metamorph." Demonstrates how to set up complex multi-dimensional imaging acquisition experiments. Reviewing multi-dimensional data sets – selecting what you need and want to see.

thumbnail preview of videoSetting up the Pro-EM to work in Metamorph

Installing and configuring the Princeton Instruments Pro-EM to work in Metamorph.

Including how to remove/install the correct PVcam driver, configuring RS config and aquaition settings inside Metamorph

thumbnail preview of videoStreaming in Metamorph

Part 3 of "Using the Multi Dimensional Acquisition in Metamorph." Demonstrates how to set up complex multi-dimensional imaging acquisition experiments. Steaming data – acquiring continual images.

thumbnail preview of videoStreamPix 4: Dual Camera Setup

Have 2 cameras and want to set them up with StreamPix 4? This step by step tutorial will help you avoid any pitfalls you might have encountered along the way. Follow along and get going.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

thumbnail preview of videoStreamPix 4: Single Camera Setup

If you’re using StreamPix 4 with your camera, this video with guide you through the setup process so that you can be up and running in no time.

 

You may also be interested in:

QImaging Application Note

Multicolor Immunofluorescence Imaging (.pdf)

thumbnail preview of videoSynchronized Camera Streaming

Tutorial of how to set up two or more cameras in StreamPix to control cameras synchronized streaming.

 

The application works by using the pulse generator to set the exposure interval and ensures both cameras are triggered simultaneously. This allows us to easily run to EMCCD cameras at 30fps for indefinite periods of time.

thumbnail preview of videoTimelapse and z Stacks in Metamorph

Part 2 of "Using the Multi Dimensional Acquisition in Metamorph." Demonstrates how to set up complex multi-dimensional imaging acquisition experiments. Control timelapse, z series, z-stacks.

thumbnail preview of videoTransferring Multiple AOIs in Image-Pro Plus

This video shows you how to use the 'Transfer Multiple AOIs' macro in Image-Pro Plus. This macro allows a user to easily save, load and interchange multiple Areas of Interest between Images in Image-Pro Plus.

thumbnail preview of videoUsing the 5D Viewer Feature of AutoQuant

This video illustrates the 3 dimensional viewer functionality of the AutoQuant deconvolution software, taking you through the basic viewing and animation options.

thumbnail preview of videoUsing the Cell Cluster Analysis Macro in Image-Pro Plus

This movie outlines the 'Cell cluster analysis' macro in Image-Pro Plus. This macro is available at Media Cybernetics Solutions Zone and allows you to easily perform a cluster analysis on images in which a regular object count proves difficult.

thumbnail preview of videoUsing the Kymograph Macro in Image-Pro Plus

This movie demonstrates the Kymograph macro of Image-Pro Plus. This macro allows you to represent a spatial position over time graphically.

thumbnail preview of videoUsing the Prior Slide Loader in Image-Pro Plus

This movie demonstrates the Prior slide loader operating inside the Image-pro Plus software using the Scope-Pro module.

thumbnail preview of videoUsing the QImaging RGB Pancake

This Video shows you how to configure and use the RGB slider 'Pancake' module with a QImaging Monochrome camera in Image-Pro Plus or Q-Capture Pro - allowing you to high resolution colour images with your monochrome camera.

thumbnail preview of videoUsing the Ringed Structure Analysis Macro in Image-Pro Plus

This video outlines the 'Perimiter region masks' macro in Image-Pro Plus, showing you how the macro can be used to measure the varying intensities of an object from the outside-inwards.

thumbnail preview of videoUsing the Vicker's Hardness Test Macro in Image-Pro Plus

This video shows you how to use the Vicker's Hardness test macro, which comes standard with Image-Pro Plus.

thumbnail preview of videoWorking with Phase Microscopy Images

This video shows you how to work with, count and measure objects in images which are difficult to effectivley threshold, using a phase microscopy as an example.


All videos execute in muted mode. To listen to the audio portion, click on the speaker button in the lower right corner of the video screen.

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